研究方向

• 方向一：对靶点或标志物明确的重大恶性肿瘤和慢性疾病，开发可用于体液诊断的便捷适配体探针（试剂盒）；对于标志物未知的重大恶性肿瘤和慢性疾病（主要是三阴性乳腺癌），以适配体探针库为抓手，通过临床样本间的对冲抵消，筛选鉴定疾病相关的标志物和对应的检测探针，揭示疾病的分子机制并明确药物可作用的靶点；

• 方向二：开发新型3D框架核酸的设计和组装方案，在保持组装体近原子分辨率的前提下，将自组装的尺度在3D空间从纳米推向微米和亚毫米级，以满足其作为介质在材料科学中的更多应用需求；同时开发活细胞生产制备框架核酸的生物技术，降低制备成本，为框架核酸的临床研究提供支持；

• 方向三：利用试管内进化的高效筛选策略分离获得重大疾病相关的核酸酶（DNAzyme和Ribozyme），将其与框架核酸融合，编辑组装“环境响应型智能纳米药物载体”，用于重大疾病的靶向精确递药。

教育工作经历

• 2022.01-          上海交通大学-化学化工学院/转化医学院，研究员

• 2015.06-2021.12     复旦大学-生物医学研究院，研究员

• 2009.11-2015.05     耶鲁大学，分子生物学，博士后，导师: Ronald Breaker院士

• 2004.08-2009.09     纽约大学，化学，博士，导师: Ned Seeman院士

• 1999.09-2004.07     中国科学技术大学，应用化学，学士，导师: 钱逸泰院士

科研任职

科研项目：

• 1、国自然基金委创新研究群体 “肿瘤免疫治疗新策略”

• 项目编号：82121002，  2022-2026  骨干 主持  200万

• 2、科技部重点研发计划 “微纳生物机器人的定向合成和诊疗应用”（课题一）

• 课题编号：2020YFA0908901，  2020-2025  主持  604万

• 3、国自然基金委重大研究计划 “肿瘤演进与诊疗的分子功能可视化研究”（培育项目）

• 项目编号：91859104，  2019-2021  主持  60万

• 4、江苏贝格尔生物医药有限公司 横向合作课题“外泌体捕捉与生物标记物检测开发”

• 2019-2020  主持  30万

• 5、国自然国际（地区）合作与交流重大项目“靶向治疗类风湿性关节炎的核酸纳米抗体研究”

• 项目编号：81861138004，  2018-2020  共同主持  总经费300万，个人承担150万

• 6、国自然面上项目 “DNA纳米骨架的组装及其在生物和材料学中的应用”

• 项目编号：21673050，  2017-2020  主持  已结题  68万

• 7、科技部国家重点研发计划“重大慢性非传染性疾病防控研究”

• 项目编号：2016YFC1306400， 2016-2020  子课题负责人  45.4万

荣誉与获奖

• 2022年上海市优秀学术带头人

代表性论文和论著

代表性文章（独立PI期间）

• Yang Yang^¥; Zhenyong Wu^¥; Laurie Wang; Kaifeng Zhou; Kai Xia; Qiancheng Xiong; Longfei Liu; Zhao Zhang; Edwin R Chapman; Yong Xiong; Thomas J Melia; Erdem Karatekin; Hongzhou Gu*; Chenxiang Lin*. “Sorting liposomes of distinct sizes by DNA-brick assisted centrifugation.” Nature Chemistry 2021, 13, 335-342.

• Youli Jia¥; Liman Chen¥; Jin Liu; Wei Li; Hongzhou Gu*. “DNA Catalyzed Efficient Production of Single-stranded DNA Nanostructures.”Chem 2021, 7, 4, 959-981.

• Qingting Li; Zongxuan Tong; Yichun Cao; Hongzhou Gu*. “DNAs catalyzing DNA nanoconstruction.” Chem 2021, 7, 10, 2556-2568.

• Canyu Zhang; Qingting Li; Tianbin Xu; Wei Li; Yungang He; Hongzhou Gu*. “New DNA-hydrolyzing DNAs isolated from an ssDNA library carrying a terminal hybridization stem.” Nucleic Acids Research 2021, 49, 11, 6364-6374.

• Xinyu Du¥; Xiaoqin Cheng¥; Wei Li; Zhilei Ge; Chunjiu Zhong; Chunhai Fan; Hongzhou Gu*. “Engineering allosteric ribozymes to detect thiamine pyrophosphate in whole blood.” Analytical Chemistry 2021, 93, 9, 4277-4284.

• Tianbin Xu¥; Canyu Zhang¥; Kai Xia; Wei Li; Yichun Cao; Hongzhou Gu*. “Small DNAs that bind nickel(II) specifically and tightly.” Analytical Chemistry 2021, DOI: 0.1021/acs.analchem.1c04034.

• Qingting Li; Shu Zhang; Wei Li; Zhilei Ge; Chunhai Fan; Hongzhou Gu*. “Programming CircLigase catalysis for DNA rings and topologies.” Analytical Chemistry 2021, 93, 1801-1810.

• Jin Liu; Hongzhou Gu*. “Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes.” STAR Protocols 2021, 2, 2, 100531.

• Huiyan Li; Fangfei Yin; Xinyi Li; Wannan Jia; Jia Ding; Li Zhang; Zhonghua Wang; Qinqin Hu; Jieliang Zuo; Huliang Jia; Lihua Wang; Chunhai Fan*; Hongzhou Gu*; Jian Wu*. “Novel aptasensor-based assay of sonic hedgehog ligand for detection of portal vein invasion of hepatocellular carcinoma.” Biosensors and Bioelectronics 2021, 174, 112738.

• Dong-Jie Tang¥; Xinyu Du¥; Qiang Shi¥; Jian-Ling Zhang¥; Yuan-Ping He; Yan-Miao Chen; Zhenhua Ming; Dan Wang; Wan-Ying Zhong; Yu-Wei Liang; Jin-Yang Liu; Jian-Ming Huang; Yun-Shi Zhong; Hongzhou Gu*; Ji-Liang Tang*. “A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA.” Nature Communications 2020, 11, 2794, 1-11.

• Kai Xia; Jianlei Shen; Qian Li; Chunhai Fan*; Hongzhou Gu*. “Near-Atomic Fabrication with Nucleic Acids.” ACS Nano 2020, 14, 1319-1337.

• Ziyi Wang; Xiao Chu; Na Li; Lingling Fu; Hongzhou Gu*; Nan Zhang*. “Engineered DNA nanodrugs alleviate inflammation in inflammatory arthritis.” International Journal of Pharmaceutics 2020, 577, 119047.

• Zongxuan Tong; Qinqin Hu; Hongzhou Gu*. “Deoxyribozymes: selection, biosensing and outlook.” Chemical Journal of Chinese Universities 2020, 41(11), 2345-2355.

• Qinqin Hu¥; Hua Li¥; Lihua Wang; Hongzhou Gu*; Chunhai Fan*. “DNA Nanotechnology Enabled Drug Delivery systems.” Chemical Reviews 2019, 119, 6459-6506.

• Hua Li; Jin Liu; Hongzhou Gu*. “Targeting Nucleolin to Obstruct Vasculature Feeding with An Intelligent DNA Nanorobot.” Journal of Cellular and Molecular Medicine 2019, 23, 2248-2250.

• Xinyu Du^¥; Xin Zhong^¥; Wei Li; Hua Li*; Hongzhou Gu*. “Retraining and Optimizing DNA-Hydrolyzing Deoxyribozymes for Robust Single- and Multiple-Turnover Activities.” ACS Catalysis 2018, 8, 5996-6005.

• Zhilei Ge^¥; Hongzhou Gu^¥; Qian Li; Chunhai Fan*. “Concept and Development of Framework Nucleic Acids.” Journal of the American Chemical Society 2018, 140, 51, 17808-17819.

• Qinqin Hu; Sheng Wang; Lihua Wang; Hongzhou Gu*; Chunhai Fan*. “DNA Nanostructure-Based Systems for Intelligent Delivery of Therapeutic Oligonucleotides.” Advanced Healthcare Materials 2018, 7, 1701153.

• Hongzhou Gu*. “Single-molecule Genotyping with Nanoscale DNA Shape IDs.” Science China Chemistry 2017, 60, 1111-1112.

代表性文章（博士-博后期间）

• Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F. Berenson; Ronald R. Breaker. “Small, Highly-active DNAs that Hydrolyze DNA.” Journal of the American Chemical Society 2013, 135, 9121-9129.

• Hongzhou Gu; Ronald R. Breaker. “Production of Single-stranded DNAs by Self-cleavage of Rolling-circle Amplification Products.” BioTechniques 2013, 54, 337-343.

• Hongzhou Gu; Kazuhiro Furukawa; Ronald R. Breaker. “Engineered Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP.” Analytical Chemistry 2012, 84, 4935-4941.

• Hongzhou Gu; Jie Chao; Shou-Jun Xiao; Nadrian C. Seeman. “A Proximity-Based Programmable DNA Nanoscale Assembly Line.” Nature 2010, 465, 202-205.

• Hongzhou Gu; Wei Yang; Nadrian C. Seeman. “DNA Scissors Device Used to Measure MutS Binding to DNA Mis-Pairs.” Journal of the American Chemical Society 2010, 132, 4352-4357.

• Hongzhou Gu; Jie Chao; Shou-Jun Xiao; Nadrian C. Seeman. “Dynamic Patterning Programmed by DNA Tiles Captured on a DNA Origami Substrate.” Nature Nanotechnology 2009, 4, 245-248.

主要专利

• 一种检测硫胺素焦磷酸的RNA传感器及其应用

专利号：201810077122.9

发明人：顾宏周、杜鑫雨、钟春玖、程小芹

• 能快速水解DNA的脱氧核酶突变体

专利号：201810131100.6

发明人：顾宏周

• 一种脱氧核酶切割方法

专利号：201810131087.4

发明人：顾宏周

• 一种长单链DNA的制备方法

专利号：201910989379.6

发明人：顾宏周、张俏

• 能快速水解DNA的II类脱氧核酶突变体

专利号：201910989374.3

发明人：顾宏周、张俏